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52978

Differential Reinforced Clostridial Broth, Base (I)


Concentration 29.00 gm/lit.
Part D
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months
HSN Code : 38210000
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
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Specifications
 
Composition :
Tryptose :10.00 gm/lit.
Yeast extract :1.50 gm/lit.
Meat extract :10.00 gm/lit.
Starch :1.00 gm/lit.
Sodium acetate,hydrated :5.00 gm/lit.
Glucose :1.00 gm/lit.
L-Cysteine hydrochloride : 0.5 gm/lit.

pH at 25 �C : 7.2 ± 0.2

Storage : Store between 8-25 �C.
Notes
  Organisms :
Clostridium sporogenes ATCC 11437
Clostridium perfringensATCC 13124

Directions :
1. Add 29.00 gm powder to distilled/deionized water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121�C for 15 minutes.
5.To 25 ml medium add 0.5 ml of filter sterilized solution prepared by
mixing equal volumes of 4% Sodium sulphite and 7% Ferric citrate.
6.To 10 ml or 50 ml Double Strength medium, add 0.4 and 2ml of the
above solution.

Applications :
Used for cultivation of Clostridia from water samples.
 
Available Packages
 
Size Price  
100 Gms
INR 710.00  
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500 Gms
INR 2870.00  
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Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
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