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74664

Bacillus Cereus Agar


Concentration 40.97 gm/lit.
Part D
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months
HSN Code : 38210000
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
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Specifications
 
Appearance (Colour)Cream to greenish yellow
Appearance (Form)Free flowing, homogeneous powder
Solubility40.97 gm/liter
Solubility before autoclaving (Clarity)Clear to slightly opalescent
Gel strength (1.5% gel)Firm, comparable with 1.5% Agar gel
pH (25°C)7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)Green.
After addition of 5.0% egg yolk emulsion: Yellowish green, opaque
Cultural ResponseInoculate and incubate at 37°C for 24 hours with the following.
OrganismBacillus cereus ATCC 10876
Inoculum (cfu) 10-100Growth : Good, Recovery rate : >=50.0%,
Colony colour :Blue,
Egg Yolk Reaction : Precipitation
OrganismStaphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100Growth : Good, Recovery rate : >=50.0%,
Colony colour : Yellow,
Egg Yolk Reaction : Clearing
OrganismProteus vulgaris ATCC 13315
Inoculum (cfu) 10-100Growth :Good, Recovery rate : >=50.0%,
Colony colour :Green,
Egg Yolk Reaction : -
OrganismEscherichia coli ATCC 25922
Inoculum>=103,Growth : Inhibited
Notes
  Applications:
Used for the isolation and enumeration of Bacillus cereus.

Composition:
Ingredients : gm/lt.
Peptone : 1.00
Mannitol : 10.00
Sodium chloride : 2.00
Magnesium sulphate : 0.10
Disodium phosphate : 2.50
Monopotassium phosphate : 0.25
Sodium pyruvate : 10.00
Bromothymol Blue : 0.12
Agar : 15.00

Directions:
1. Add 20.50 gm powder to 475.0 ml distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 50°C. Aseptically add 25 ml of sterile egg yolk emulsion and rehydrated contents of 1 vial of Polymixin B Selective Supplement. (35980).
 
Available Packages
 
Size Price  
100 Gms
INR 880.00  
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500 Gms
INR 3894.00  
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Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
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