Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
25.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18- 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 23724
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli DH5 alpha
MTCC 1652 50-100 luxurian
Inoculum (cfu) 10-100
Growth : Good
Applications
Used for maintaining and propagating Escherichia coli in molecular microbiology procedures.
Composition
Ingredients
gm/lt.
Veg. Hydrolysate
10.00
Yeast extract
5.00
Sodium chloride
10.00
Directions
1. Add 25.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into test tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
