Appearance (Colour)
Yellow
Appearance (Form)
Crystalline powder
Solubility (Turbidity) 5% solution in Methanol
Clear
Solubility (Colour) 5% solution in methanol
Yellow
Assay
min. 98%
Absorbance (A) of 1% solution in Methanol in a 1cm cell
@242nm
400 - 530
Application
MTT is utilized for the detection of cell viability and cell proliferation studies. Since MTT has a positive charge, it can pass through a cell membrane and is reduced by mitochondria to form a purple color formazan dye. Organic solvents are required to dissovle MTT formazan dyes. MTT is also rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
