Appearance (Colour)
Light yellow, granular powder
Clarity of solution
Clear to very slightly opalescent
Solubility
Solubility at 12% : Aqueous solution is clear at 55°C and also after autoclave at 15Ibs pressure at 121°C for 15 minutes
pH
4.5 - 6.3 ( 1% a.q ) at 55°C
Gel strength
~ 120g Bloom
Stability (After autoclaving)
Light amber, clear to very slightly opalescent
Loss on drying
<= 16.0%
Ash
< 3.25%
Sulphur dioxide
< 0.02%
Nitrogen (N)
~ 15.0% -16.0%
Cultural Response
Inoculate and incubate at 37°C for 18-48 hours or upto 2 weeks for the gelatinase tests with the following organisms
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good, Gelatinase activity : Positive
Organism
Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100
Growth : Good, Gelatinase activity : Positive
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Gelatinase activity : Negative
Applications
Gelatin is a collagenous protein which finds its application for the solidification of cultural media. It is also used for detection and differentiation of certain proteolytic bacteria. A bacteriological grade, readily soluble in water.
